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 ผลงานตีพิมพ์ในวารสารวิชาการDevelopment of specific simple sequence repeat (SSR) markers for non-pollen type thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)ผู้แต่ง: Matthayatthaworn, W.,  Dr.Prapa Sripichitt, Associate Professor , Dr.Chalermpol Phumichai, Associate Professor , Dr.Sarawut Rungmekarat, Assistant Professor , Uckarach, S., Dr.Tanee Sreewongchai, Associate Professor , วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการCross-genera transferability of (simple sequence repeat) SSR markers among cassava (Manihotesculenta crantz), rubber tree (Hevea brasiliensis muell. Arg.) and physic nut (Jatropha curcas L.)ผู้แต่ง:Whankaew, S, Kanjanawattanawong, S., Dr.Chalermpol Phumichai, Associate Professor, Smith, D.R.a, Narangajavana, J., Triwitayakorn, K., วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Assessment of Genetic Diversity and Population Structure in Jute (Corchorus spp.) Using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) Marker ผู้เขียน:Ranjit Kumar Ghosh, อรุณี วงษ์แก้ว, ดร.ธานี ศรีวงศ์ชัย, รองศาสตราจารย์, ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์ , ดร.เฉลิมพล ภูมิไชย์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe genetic diversity and population structure of 63 jute genotypes from C. capsularis L. and C. olitorius L. were investigated using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The average polymorphism information content (PIC) value for the SSR and AFLP marker systems was 0.41 and 0.47, respectively. Primer MJM136 for SSR and primer combination E-AGG/M-CTA for AFLP showed the highest PIC values (0.51 and 0.50, respectively), indicating they were the most informative primers for the assessment of genetic diversity in jute and that SSR and AFLP markers are useful for distinguishing jute genotypes. The PIC value for C. olitorius was relatively higher (0.45) than for C. capsularis (0.43). Cluster analysis based on the unweighted pair group method of arithmetic means clearly classified the genotypes of the two jute species into two main clusters. The results from the analysis of molecular variance revealed 81% molecular variation between species but it was low (19%) within species. The most diverse genotypes were identified as IND4546, TAN4231 and BRA4794 in C. olitorius and CVL-1, BAN2596C and CHI4995C in C. capsularis and these could be used as the most diverse genetic material in future breeding programs for jute improvement. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Detection of Quality Protein Maize (QPM) using Simple Sequence Repeat (SSR) Markers and Analysis of Tryptophan Content in Endosperm) ผู้เขียน:ดร.พีรนุช จอมพุก, รองศาสตราจารย์ , ดร.วาสนา วงษ์ใหญ่, รองศาสตราจารย์ , ดร.ชบา จำปาทอง, Somsak Apisitvanich สื่อสิ่งพิมพ์:pdf AbstractQuality protein maize (QPM) controlled by opaque-2 (o2) gene was detected using two simple sequence repeat (SSR) markers and further confirmed by tryptophan content in the endosperm. Three populations, i.e., Pop61C1, Pop62C6 and Pop65C6 from the International Maize and Wheat Improvement Center (CIMMYT) were used. S0-plants were selected for the presence of QPM using two markers (phi057 and phi112) as indicated by the amplified products of 140-160 bp. The phi057 marker identified 24 out of 40 Pop61C1 plants (60%), 34 out of 35 Pop62C6 plants (97%) and 24 out of 30 Pop65C6 plants (80%) to be opaque-2 positive while phi112 marker identified 34 (85%), 35 (100%) and 30 (100%) to be opaque-2 plants from the respective populations. Since phi112 was a dominant marker not for detecting the heterozygous genotype, it could only indicate the difference between QPM inbreds and normal maize. The phi057, on the other hand, is a co-dominant marker and could identify the heterozygote of maize plants and therefore, the contamination of non-QPM presented in CIMMYT population. The endosperms of selected S1-seed were further analyzed for the tryptophan and protein content. The three maize populations were found containing the same protein quantity, but different in tryptophan content. The average tryptophan contents in endosperm of QPM and non-QPM as detected by phi057 marker were 0.66% (for QPM), 0.38% (for non-QPM), 0.38% for Suwan 1 (a non-QPM) and 0.80% for the opaque-2 control variety. Moreover, those QPM and non-QPM plants detected by phi112 showed the same result of total protein and tryptophan content in endosperm. To detect heterozygote maize for backcross breeding program, phi057 was considered more feasible than phi112 as a marker assisted selection (MAS) for opaque-2 and to identify QPM line in the short period of time. |
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ที่มา:สำนักงานกองทุนสนับสนุนการวิจัยหัวเรื่อง:การประเมินความหลากหลายทางพันธุกรรมของส้มโอพันธุ์การค้าและพันธุ์พื้นเมืองในประเทศไทยด้วยเครื่องหมาย Simple Sequence Repeat(SSR) หัวหน้าโครงการ:ดร.อุณารุจ บุญประกอบ, รองศาสตราจารย์ |
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ผลงานตีพิมพ์ในวารสารวิชาการSSR-based and grain yield-based diversity of hybrid maize in Thailandผู้แต่ง:Dr.Chalermpol Phumichai, Associate Professor, W. Doungchan, P.Puddhanon, Dr.Sansern Jampatong , P Grudloyma, C. Kirdsri, Dr.Julapark Chunwongse, Associate Professor , T. Pulam, วารสาร:  |
ผลงานตีพิมพ์ในวารสารวิชาการQTL mapping of pericarp thickness in immature and mature stages in thai tropical sweet corn (Zea mays var. saccharata)ผู้แต่ง:Wanlayaporn, K., Somyong, S., Pootakham, W., Shearman, J., Dr.Apichart Vanavichit, Professor , Kumar, P., Chee, P.W., Tragoonrung, S., วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการGenetic diversity of zombi pea (Vigna vexillata) assessed by microsatellite markersผู้แต่ง:Dr.Prakit Somta, Associate Professor , Dachapak, S., Yimram, T., Dr.Peerasak Srinives, Professor , Poonchaivilaisak, S., วารสาร: |
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